Review




Structured Review

Novoprotein soluble trail protein
A: Blank control group; B: 5 µmol/L paclitaxel positive control group; C: 3.12 <t>µmol/L</t> <t>oridonin;</t> D: 6.25 µmol/L oridonin; E: 12.5 µmol/L oridonin; F: 31.2 ng/mL <t>TRAIL;</t> G: 62.5 ng/mL TRAIL; H: 125 ng/mL TRAIL; I: 3.12 µmol/L oridonin+31.2 ng/mL TRAIL; J: 6.25 µmol/L oridonin+62.5 ng/mL TRAIL; K: 12.5 µmol/L oridonin+125 ng/mL TRAIL.
Soluble Trail Protein, supplied by Novoprotein, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/soluble trail protein/product/Novoprotein
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Images

1) Product Images from "Combination of oridonin and TRAIL induces apoptosis in uveal melanoma cells by upregulating DR5"

Article Title: Combination of oridonin and TRAIL induces apoptosis in uveal melanoma cells by upregulating DR5

Journal: International Journal of Ophthalmology

doi: 10.18240/ijo.2021.12.05

A: Blank control group; B: 5 µmol/L paclitaxel positive control group; C: 3.12 µmol/L oridonin; D: 6.25 µmol/L oridonin; E: 12.5 µmol/L oridonin; F: 31.2 ng/mL TRAIL; G: 62.5 ng/mL TRAIL; H: 125 ng/mL TRAIL; I: 3.12 µmol/L oridonin+31.2 ng/mL TRAIL; J: 6.25 µmol/L oridonin+62.5 ng/mL TRAIL; K: 12.5 µmol/L oridonin+125 ng/mL TRAIL.
Figure Legend Snippet: A: Blank control group; B: 5 µmol/L paclitaxel positive control group; C: 3.12 µmol/L oridonin; D: 6.25 µmol/L oridonin; E: 12.5 µmol/L oridonin; F: 31.2 ng/mL TRAIL; G: 62.5 ng/mL TRAIL; H: 125 ng/mL TRAIL; I: 3.12 µmol/L oridonin+31.2 ng/mL TRAIL; J: 6.25 µmol/L oridonin+62.5 ng/mL TRAIL; K: 12.5 µmol/L oridonin+125 ng/mL TRAIL.

Techniques Used: Control, Positive Control

A-G: Flow cytometry analysis of cell apoptosis: A: Blank control group; B: 3.12 µmol/L oridonin; C: 31.2 ng/mL TRAIL; D: 3.12 µmol/L oridonin+31.2 ng/mL TRAIL; E: 12.5 µmol/L oridonin; F: 125 ng/mL TRAIL; G: 12.5 µmol/L oridonin+ 125 ng/mL TRAIL; H: Histogram present the proportion of apoptotic cells in each group, aP<0.05, bP<0.01, compared with control group.
Figure Legend Snippet: A-G: Flow cytometry analysis of cell apoptosis: A: Blank control group; B: 3.12 µmol/L oridonin; C: 31.2 ng/mL TRAIL; D: 3.12 µmol/L oridonin+31.2 ng/mL TRAIL; E: 12.5 µmol/L oridonin; F: 125 ng/mL TRAIL; G: 12.5 µmol/L oridonin+ 125 ng/mL TRAIL; H: Histogram present the proportion of apoptotic cells in each group, aP<0.05, bP<0.01, compared with control group.

Techniques Used: Flow Cytometry, Control

A: The relative expression level of DR5 gene after transfection with DR5 siRNA was detected by qPCR; B: The effect of TRAIL and oridonin on the activity of transfected si-DR5 cells was detected by MTT; C: Western blot was used to detect the expression levels of apoptosis-related proteins in each group; D: A histogram obtained by quantizing the gray value of the protein band in C. aStatistically significant difference compared with the control group, P<0.05.
Figure Legend Snippet: A: The relative expression level of DR5 gene after transfection with DR5 siRNA was detected by qPCR; B: The effect of TRAIL and oridonin on the activity of transfected si-DR5 cells was detected by MTT; C: Western blot was used to detect the expression levels of apoptosis-related proteins in each group; D: A histogram obtained by quantizing the gray value of the protein band in C. aStatistically significant difference compared with the control group, P<0.05.

Techniques Used: Expressing, Transfection, Activity Assay, Western Blot, Control



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A: Blank control group; B: 5 µmol/L paclitaxel positive control group; C: 3.12 <t>µmol/L</t> <t>oridonin;</t> D: 6.25 µmol/L oridonin; E: 12.5 µmol/L oridonin; F: 31.2 ng/mL <t>TRAIL;</t> G: 62.5 ng/mL TRAIL; H: 125 ng/mL TRAIL; I: 3.12 µmol/L oridonin+31.2 ng/mL TRAIL; J: 6.25 µmol/L oridonin+62.5 ng/mL TRAIL; K: 12.5 µmol/L oridonin+125 ng/mL TRAIL.
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Image Search Results


A: Blank control group; B: 5 µmol/L paclitaxel positive control group; C: 3.12 µmol/L oridonin; D: 6.25 µmol/L oridonin; E: 12.5 µmol/L oridonin; F: 31.2 ng/mL TRAIL; G: 62.5 ng/mL TRAIL; H: 125 ng/mL TRAIL; I: 3.12 µmol/L oridonin+31.2 ng/mL TRAIL; J: 6.25 µmol/L oridonin+62.5 ng/mL TRAIL; K: 12.5 µmol/L oridonin+125 ng/mL TRAIL.

Journal: International Journal of Ophthalmology

Article Title: Combination of oridonin and TRAIL induces apoptosis in uveal melanoma cells by upregulating DR5

doi: 10.18240/ijo.2021.12.05

Figure Lengend Snippet: A: Blank control group; B: 5 µmol/L paclitaxel positive control group; C: 3.12 µmol/L oridonin; D: 6.25 µmol/L oridonin; E: 12.5 µmol/L oridonin; F: 31.2 ng/mL TRAIL; G: 62.5 ng/mL TRAIL; H: 125 ng/mL TRAIL; I: 3.12 µmol/L oridonin+31.2 ng/mL TRAIL; J: 6.25 µmol/L oridonin+62.5 ng/mL TRAIL; K: 12.5 µmol/L oridonin+125 ng/mL TRAIL.

Article Snippet: Soluble TRAIL protein (Novoprotein, Shanghai); oridonin (Chengdu Institute of Biology, Chinese Academy of Sciences); MTT (AMRESCO); RPMI1640 (Thermo Fisher); foetal bovine serum (FBS, GIBCO); DMSO (Life science); paclitaxel (Shanghai Yuanye Bio-Technology); penicillin (Life Technology); Streptomycin (GIBCO); DR5 antibody (Abcam); a-caspase-3 antibody (Abcam); x-linked inhibitor of apoptosis protein (XIAP) antibody (Abcam); β-actin antibody (Abcam).

Techniques: Control, Positive Control

A-G: Flow cytometry analysis of cell apoptosis: A: Blank control group; B: 3.12 µmol/L oridonin; C: 31.2 ng/mL TRAIL; D: 3.12 µmol/L oridonin+31.2 ng/mL TRAIL; E: 12.5 µmol/L oridonin; F: 125 ng/mL TRAIL; G: 12.5 µmol/L oridonin+ 125 ng/mL TRAIL; H: Histogram present the proportion of apoptotic cells in each group, aP<0.05, bP<0.01, compared with control group.

Journal: International Journal of Ophthalmology

Article Title: Combination of oridonin and TRAIL induces apoptosis in uveal melanoma cells by upregulating DR5

doi: 10.18240/ijo.2021.12.05

Figure Lengend Snippet: A-G: Flow cytometry analysis of cell apoptosis: A: Blank control group; B: 3.12 µmol/L oridonin; C: 31.2 ng/mL TRAIL; D: 3.12 µmol/L oridonin+31.2 ng/mL TRAIL; E: 12.5 µmol/L oridonin; F: 125 ng/mL TRAIL; G: 12.5 µmol/L oridonin+ 125 ng/mL TRAIL; H: Histogram present the proportion of apoptotic cells in each group, aP<0.05, bP<0.01, compared with control group.

Article Snippet: Soluble TRAIL protein (Novoprotein, Shanghai); oridonin (Chengdu Institute of Biology, Chinese Academy of Sciences); MTT (AMRESCO); RPMI1640 (Thermo Fisher); foetal bovine serum (FBS, GIBCO); DMSO (Life science); paclitaxel (Shanghai Yuanye Bio-Technology); penicillin (Life Technology); Streptomycin (GIBCO); DR5 antibody (Abcam); a-caspase-3 antibody (Abcam); x-linked inhibitor of apoptosis protein (XIAP) antibody (Abcam); β-actin antibody (Abcam).

Techniques: Flow Cytometry, Control

A: The relative expression level of DR5 gene after transfection with DR5 siRNA was detected by qPCR; B: The effect of TRAIL and oridonin on the activity of transfected si-DR5 cells was detected by MTT; C: Western blot was used to detect the expression levels of apoptosis-related proteins in each group; D: A histogram obtained by quantizing the gray value of the protein band in C. aStatistically significant difference compared with the control group, P<0.05.

Journal: International Journal of Ophthalmology

Article Title: Combination of oridonin and TRAIL induces apoptosis in uveal melanoma cells by upregulating DR5

doi: 10.18240/ijo.2021.12.05

Figure Lengend Snippet: A: The relative expression level of DR5 gene after transfection with DR5 siRNA was detected by qPCR; B: The effect of TRAIL and oridonin on the activity of transfected si-DR5 cells was detected by MTT; C: Western blot was used to detect the expression levels of apoptosis-related proteins in each group; D: A histogram obtained by quantizing the gray value of the protein band in C. aStatistically significant difference compared with the control group, P<0.05.

Article Snippet: Soluble TRAIL protein (Novoprotein, Shanghai); oridonin (Chengdu Institute of Biology, Chinese Academy of Sciences); MTT (AMRESCO); RPMI1640 (Thermo Fisher); foetal bovine serum (FBS, GIBCO); DMSO (Life science); paclitaxel (Shanghai Yuanye Bio-Technology); penicillin (Life Technology); Streptomycin (GIBCO); DR5 antibody (Abcam); a-caspase-3 antibody (Abcam); x-linked inhibitor of apoptosis protein (XIAP) antibody (Abcam); β-actin antibody (Abcam).

Techniques: Expressing, Transfection, Activity Assay, Western Blot, Control

FIGURE 5 IL-18/poly I:C-primed NK cells kill hepatic stellate cells in a TRAIL- involved degranulation manner. IL-18 and poly I:C-primed NK cells were treated by anti-TRAIL or isotype antibody before coculture. HSC death was measured (A), and NK cell degranulation was evaluated by CD107a expression (C). Soluble TRAIL was added to cell coculture, the cell death of HSCs (B) and CD107a expression of NK cells (D) was shown. Results were shown as mean ± SEM of 6 independent experiments performed with 6 different donor NK cells. *P < 0.05, **P < 0.01, ***P < 0.001, paired t-test. The kinetics of the activated NK cell-mediated LX2 cell apoptosis were observed by using live-cell imaging (E).

Journal: Journal of leukocyte biology

Article Title: Activated NK cells kill hepatic stellate cells via p38/PI3K signaling in a TRAIL-involved degranulation manner.

doi: 10.1002/JLB.2A0118-031RR

Figure Lengend Snippet: FIGURE 5 IL-18/poly I:C-primed NK cells kill hepatic stellate cells in a TRAIL- involved degranulation manner. IL-18 and poly I:C-primed NK cells were treated by anti-TRAIL or isotype antibody before coculture. HSC death was measured (A), and NK cell degranulation was evaluated by CD107a expression (C). Soluble TRAIL was added to cell coculture, the cell death of HSCs (B) and CD107a expression of NK cells (D) was shown. Results were shown as mean ± SEM of 6 independent experiments performed with 6 different donor NK cells. *P < 0.05, **P < 0.01, ***P < 0.001, paired t-test. The kinetics of the activated NK cell-mediated LX2 cell apoptosis were observed by using live-cell imaging (E).

Article Snippet: Soluble TRAIL (R&D System; Catalog number: 375-TL-010) was added to HSCs half hour earlier than NK cells in coculture.

Techniques: Expressing, Live Cell Imaging

FIGURE 6 IL-18/poly I:C-primed hepatic NK cells kill HSCs in a TRAIL-involved degranulation manner. Liver NK cells were purified from speci- men, then primed by IL-18 and/or poly I:C before cocultured with primary HSCs as above. CD107a expression of liver NK cells (A) and PI/AnnexinV level of primary HSCs (B) were detected. Anti-TRAIL or isotype antibody were used to treat IL-18/poly I:C-primed liver NK cells before cocultured with primary HSCs, then CD107a expression of liver NK cells (C) and the cell death of primary HSCs (D) was measured. Results were shown as mean ± SEM of 5 independent experiments performed with 5 different donor NK cells. *P < 0.05, **P < 0.01, paired t-test.

Journal: Journal of leukocyte biology

Article Title: Activated NK cells kill hepatic stellate cells via p38/PI3K signaling in a TRAIL-involved degranulation manner.

doi: 10.1002/JLB.2A0118-031RR

Figure Lengend Snippet: FIGURE 6 IL-18/poly I:C-primed hepatic NK cells kill HSCs in a TRAIL-involved degranulation manner. Liver NK cells were purified from speci- men, then primed by IL-18 and/or poly I:C before cocultured with primary HSCs as above. CD107a expression of liver NK cells (A) and PI/AnnexinV level of primary HSCs (B) were detected. Anti-TRAIL or isotype antibody were used to treat IL-18/poly I:C-primed liver NK cells before cocultured with primary HSCs, then CD107a expression of liver NK cells (C) and the cell death of primary HSCs (D) was measured. Results were shown as mean ± SEM of 5 independent experiments performed with 5 different donor NK cells. *P < 0.05, **P < 0.01, paired t-test.

Article Snippet: Soluble TRAIL (R&D System; Catalog number: 375-TL-010) was added to HSCs half hour earlier than NK cells in coculture.

Techniques: Purification, Expressing